Toxicology

The Bionas DiscoveryTM 2500 system offers an alternative approach to animal testing to assess the toxicological risks of soluble chemicals and xenobiotics. It is the only commercially available system combining three crucial parameters for the study of cell physiology.
Under typical in vitro cell culture conditions, oxygen consumption rate is a direct measurement of mitochondrial respiration. The extracellular acidification rate is dominated by lactic acid production during glycolytic energy metabolism. By generating real-time profiles of O2 consumption and extracellular acidification which both determine the acute rates of the cellular energy metabolism, the multi-parameter measurement allows the monitoring of the actions of substances and their cytotoxic effects including potential regeneration.


 

Effect of respiratory chain inhibitor


Fig. Tox 1:

Effect of respiratory chain inhibitor: Real-time measurement of acidification and respiration rates and impedance/adhesion of CHO cells treated with KCN showing a rapid reduction of the respiration rate of 50% while the acidification rate increases by 25%. Impedance/adhesion is only marginally influenced. After withdrawal of KCN a complete regeneration is seen.
Fig. Tox 2:
Effect of glycolysis and glucose transport inhibitor: Real-time measurement of acidification and respiration rates and impedance/adhesion of V79 cells treated with cytochalasin B. The inhibition of glucose transport through the membrane and actin polymerization is detected in real-time. At the same time respiration rates are increased indicating the compensation of reduced ATP production via glycolysis. All three parameters show almost complete regeneration when cytochalasin B is removed after 3 hrs.
Fig. Tox 3A:

Real-time monitoring of respiration of rat primary hepatocytes treated with APAP shows a concentration dependent inhibition/reduction of all three parameters. Not all parameters recover to the initial base levels indicating an irreversible damage of the cells by APAP.

Continued below...
Fig. Tox 3B:

... continued from above.

Real-time monitoring of acidification rates.
Fig. Tox 3C:

... continued from above.

Real-time monitoring of impedance/adhesion.