Acidification Rate

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On-line control of extracellular acidification with pH ISFET sensors

The extra-cellular acidification rate is closely linked to cellular energy metabolism (glycolysis). Cells develop an acidic extra cellular environment as a result of breaking down nutrients into energy and waste products. The primary waste products are lactic acid and carbonic acid. They occur during aerobic and anaerobic glycolysis.
This means that acidification is closely linked to the rate at which cells convert nutrients to energy.
SEM micrographs of murine embryonic neuronal cells on the sensitive area of ISFET. Growth and adhesion of the cells are not influenced by the sensors.