Adhesion & Confluence

 

On-line control of cellular adhesion & confluence with impedance measurements using IDES sensors

Adhesion and changes in adhesion or cell morphology are key features in the life of a cell. Adhesive interactions enable individual cells to form and stabilize close contacts in order to maintain higher-order tissue specialization and facilitate information transfer.

In vitro adherently growing cells interact with their extra cellular environment by means of glycoproteins and other glycoconjugates. The cells spread out until a cell-cell contact is reached.

The main effect of cells on IDES is due to the insulating property of the cell membrane. The presence of intact cell membranes on the electrodes determines the current flow and thus the sensor signal.

The IDES sensor is integrated in the metabolic chip Bionas DisocveryTM SC1000.
The integration of IDES sensors in a multiwell application has been realized in the Bionas DiscoveryTM adcon plate.

SEM micrographs of murine embryonic neuronal cells on the sensitive area of IDES. Growth and adhesion of the cells are not influenced by the sensors.